Publication Date

1985

Location

Kyoto Japan

Description

Successful use of near infrared reflectance spectroscopy (NIRS) analysis of crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF) of farm-grown alfalfa (Medicago sativa) is described. Calibration equations for CP, ADF, and NDF using the BEST/NeoTech 6100 IR spectro-computer system1 were developed from windrow alfalfa samples collected throughout Minnesota over three seasons. Standard errors of calibration were 0.57, 1.56 and 1.83 dag/kg for CP, ADF and NDF, respectively. Coefficients of multiple determination (R2) were 0.97, 0. 91 and 0. 93, respectively. Standard errors of NIRS analysis for CP, ADF, and NDF were 0.58, 1.48 and 1.71, respectively. Squared simple correlation coefficients between NIRS and conventional values were 0.97, 0.93 and 0.95, respectively. We conclude that the accuracy and speed of NIRS analysis of farm-grown alfalfa, coupled with forage quality standards, are needed and accepted by farmers. This technologic advance will promote use of improved quality forages and lead to improved livestock performance.

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Near Infrared Reflectance Spectroscopy Analysis of Quality of Farm- Grown Alfalfa

Kyoto Japan

Successful use of near infrared reflectance spectroscopy (NIRS) analysis of crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF) of farm-grown alfalfa (Medicago sativa) is described. Calibration equations for CP, ADF, and NDF using the BEST/NeoTech 6100 IR spectro-computer system1 were developed from windrow alfalfa samples collected throughout Minnesota over three seasons. Standard errors of calibration were 0.57, 1.56 and 1.83 dag/kg for CP, ADF and NDF, respectively. Coefficients of multiple determination (R2) were 0.97, 0. 91 and 0. 93, respectively. Standard errors of NIRS analysis for CP, ADF, and NDF were 0.58, 1.48 and 1.71, respectively. Squared simple correlation coefficients between NIRS and conventional values were 0.97, 0.93 and 0.95, respectively. We conclude that the accuracy and speed of NIRS analysis of farm-grown alfalfa, coupled with forage quality standards, are needed and accepted by farmers. This technologic advance will promote use of improved quality forages and lead to improved livestock performance.