Theme 15: Advances in Rhizobial Research
Phylogenetic Grouping by PCR Analyses of Sinorhizobium meliloti Strains Isolated from Eutrophic Soil
Description
This study evaluated the ability of Sinorhizobium meliloti strains (SEMIA-116, SEMIA- 134 and SEMIA-135) to persist in eutrophic soils after three complete growing cycles of alfalfa. PCR fingerprinting using primers for the nifH, Eric, Rep, RP genes and P25 RAPD-PCR performed the phylogenetic grouping of the three commercial strains and 39 field isolates. These analyzes were sufficiently sensitive to discriminate and group the strains and isolate. Our preliminary studies were based upon Eric primers and RP01 RAPD-PCR.
Citation
Oliveira, W. S.; Oliveira, P. P. A.; Bellato, C. M.; Duarte, F. R. S.; and Tsai, S. M., "Phylogenetic Grouping by PCR Analyses of Sinorhizobium meliloti Strains Isolated from Eutrophic Soil" (2021). IGC Proceedings (1993-2023). 3.
https://uknowledge.uky.edu/igc/19/15/3
Included in
Phylogenetic Grouping by PCR Analyses of Sinorhizobium meliloti Strains Isolated from Eutrophic Soil
This study evaluated the ability of Sinorhizobium meliloti strains (SEMIA-116, SEMIA- 134 and SEMIA-135) to persist in eutrophic soils after three complete growing cycles of alfalfa. PCR fingerprinting using primers for the nifH, Eric, Rep, RP genes and P25 RAPD-PCR performed the phylogenetic grouping of the three commercial strains and 39 field isolates. These analyzes were sufficiently sensitive to discriminate and group the strains and isolate. Our preliminary studies were based upon Eric primers and RP01 RAPD-PCR.
