Theme 15: Advances in Rhizobial Research
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Phylogenetic Grouping by PCR Analyses of Sinorhizobium meliloti Strains Isolated from Eutrophic Soil
Publication Date
2001
Location
Brazil
Description
This study evaluated the ability of Sinorhizobium meliloti strains (SEMIA-116, SEMIA- 134 and SEMIA-135) to persist in eutrophic soils after three complete growing cycles of alfalfa. PCR fingerprinting using primers for the nifH, Eric, Rep, RP genes and P25 RAPD-PCR performed the phylogenetic grouping of the three commercial strains and 39 field isolates. These analyzes were sufficiently sensitive to discriminate and group the strains and isolate. Our preliminary studies were based upon Eric primers and RP01 RAPD-PCR.
Citation
Oliveira, W. S.; Oliveira, P. P. A.; Bellato, C. M.; Duarte, F. R. S.; and Tsai, S. M., "Phylogenetic Grouping by PCR Analyses of Sinorhizobium meliloti Strains Isolated from Eutrophic Soil" (2001). IGC Proceedings (1985-2023). 3.
(URL: https://uknowledge.uky.edu/igc/19/15/3)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Phylogenetic Grouping by PCR Analyses of Sinorhizobium meliloti Strains Isolated from Eutrophic Soil
Brazil
This study evaluated the ability of Sinorhizobium meliloti strains (SEMIA-116, SEMIA- 134 and SEMIA-135) to persist in eutrophic soils after three complete growing cycles of alfalfa. PCR fingerprinting using primers for the nifH, Eric, Rep, RP genes and P25 RAPD-PCR performed the phylogenetic grouping of the three commercial strains and 39 field isolates. These analyzes were sufficiently sensitive to discriminate and group the strains and isolate. Our preliminary studies were based upon Eric primers and RP01 RAPD-PCR.
