Positioning of the SCRAMBLED Receptor Requires UDP-Glc:sterol Glucosyltransferase 80B1 in Arabidopsis Roots

Authors

Victoria G. Pook, University of KentuckyFollow
Meera Nair, University of KentuckyFollow
KookHui Ryu, University of Michigan
James C. Arpin, Kansas State University
John Schiefelbein, University of Michigan
Kathrin Schrick, Kansas State University
Seth DeBolt, University of KentuckyFollow

Abstract

The biological function of sterol glucosides (SGs), the most abundant sterol derivatives in higher plants, remains uncertain. In an effort to improve our understanding of these membrane lipids we examined phenotypes exhibited by the roots of Arabidopsis (Arabidopsis thaliana) lines carrying insertions in the UDP-Glc:sterol glucosyltransferase genes, UGT80A2 and UGT80B1. We show that although ugt80A2 mutants exhibit significantly lower levels of total SGs they are morphologically indistinguishable from wild-type plants. In contrast, the roots of ugt80B1 mutants are only deficient in stigmasteryl glucosides but exhibit a significant reduction in root hairs. Sub-cellular investigations reveal that the plasma membrane cell fate regulator, SCRAMBLED (SCM), is mislocalized in ugt80B1 mutants, underscoring the aberrant root epidermal cell patterning. Live imaging of roots indicates that SCM:GFP is localized to the cytoplasm in a non cell type dependent manner instead of the hair (H) cell plasma membrane in these mutants. In addition, we provide evidence for the localization of the UGT80B1 enzyme in the plasma membrane. These data lend further support to the notion that deficiencies in specific SGs are sufficient to disrupt normal cell function and point to a possible role for SGs in cargo transport and/or protein targeting to the plasma membrane.

Document Type

Article

Publication Date

7-18-2017

Notes/Citation Information

Published in Scientific Reports, v. 7, article number: 5714, p. 1-10.

© The Author(s) 2017

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Digital Object Identifier (DOI)

https://doi.org/10.1038/s41598-017-05925-6

Funding Information

National Science Foundation MCB-1122016 (KS), IOS-1444400 (JS) and Cooperative Agreement No. 1355438 (SD) as well as United States Department of Agriculture Hatch funding also supported this work (VGP). JCA was supported by the Kansas IDeA Network of Biomedical Research Excellence (National Institutes of Health P20GM103418).

Related Content

Supplementary information accompanies this paper at doi: 10.1038/s41598-017-05925-6

Repository Citation

Pook, Victoria G.; Nair, Meera; Ryu, KookHui; Arpin, James C.; Schiefelbein, John; Schrick, Kathrin; and DeBolt, Seth, "Positioning of the SCRAMBLED Receptor Requires UDP-Glc:sterol Glucosyltransferase 80B1 in Arabidopsis Roots" (2017). Horticulture Faculty Publications. 26.
https://uknowledge.uky.edu/horticulture_facpub/26