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Author ORCID Identifier
Date Available
4-15-2022
Year of Publication
2022
Document Type
Master's Thesis
Degree Name
Master of Science (MS)
College
Agriculture, Food and Environment
Department/School/Program
Veterinary Science
Faculty
Dr. Scott D. Stanley
Faculty
Dr. Martin Nielsen
Abstract
Bone resorptive diseases affect humans and horses, alike. Examples of these diseases include osteoporosis, Paget’s disease, and osteogenesis imperfecta in humans, and navicular disease in the horse (Mitchell et al., 2019; Suva et al., 2021). Bisphosphonates are used to treat these diseases, and may remain in the bone for several years after administration, demonstrating the need for newer drug testing methods.
The purpose of the discovery phase of this study was to use blood samples of in-training horses which had been given an initial administration of the bisphosphonate tiludronate and identify protein biomarkers that changed in response to this class of drugs. In the targeted phase, the objective was to validate the previously identified protein biomarkers that can be added into a biomarker library. This library is meant to be referenced when drug testing racehorses and can lengthen the detection window of prohibited or restricted substances.
Fractionation, digestion, and de-salting were performed on sera samples from 19 equine subjects in-training. These samples were injected in an Orbitrap™ Exploris 480d coupled with an Ultimate 3000 RSLCnanoj. Label-free quantitation of proteins was performed in both phases. Four key protein biomarkers were identified in the discovery phase and validated in the targeted phase.
Digital Object Identifier (DOI)
https://doi.org/10.13023/etd.2022.044
Recommended Citation
Porter, Malinda Jayne Alison, "Identifying Bisphosphonate Protein Biomarkers in Equine Sera Using Mass Spectrometry Methods" (2022). Theses and Dissertations--Veterinary Science. 54.
https://uknowledge.uky.edu/gluck_etds/54
