Meta-Analysis of Cyathostomin Species-Specific Prevalence and Relative Abundance in Domestic Horses from 1975-2020: Emphasis on Geographical Region and Specimen Collection Method
BACKGROUND: Cyathostomins infect virtually all horses, and concomitant infections with 10 or more species per horse is standard. Species-specific knowledge is limited, despite potential species bias in development of disease and anthelmintic resistance. This is the first meta-analysis to examine effects of geographical region and cyathostomin collection method on reported composition of cyathostomin communities.
METHODS: Thirty-seven articles published in English in 1975 or later, in which adults of individual species were systematically enumerated, were included. Seven regions; North America, South America, eastern Europe, western Europe, northern Europe, southern Africa, and Oceania, and three cyathostomin collection methods; (i) standard necropsy recovery from the large intestine, (ii) critical test collection from post-treatment feces and necropsy, and (iii) diagnostic deworming recovery solely from post-treatment feces, were considered. Generalized mixed linear models analyzed the effects of region and collection method on species-specific prevalence and relative abundance. Species richness was analyzed by mixed linear models.
RESULTS: Definitively, the most prevalent and relatively abundant species were Cylicocyclus nassatus (prevalence = 93%, relative abundance = 20%), Cylicostephanus (Cys.) longibursatus (93%, 20%), and Cyathostomum catinatum (90%, 16%). A bias toward horses with high infection intensities and cyathostomin collection from feces resulted in North American critical tests and eastern European diagnostic deworming overestimating the species-specific prevalence and underestimating the relative abundance of rare/uncommon species compared to respective intra-regional standard necropsies. North American critical tests underestimated species richness due partially to identification key errors. Inter-regional standard necropsy comparisons yielded some species-specific regional differences, including a significantly higher Cys. longibursatus prevalence and relative abundance in North America (92%, 33%) than in eastern Europe (51%, 7%) (P > 0.0001). Localization of critical tests to North America and diagnostic deworming to Eastern Europe precluded expansive ‘region by collection method’ interaction analyses.
CONCLUSION: We provide substantial data to inform study design, e.g. effect and study size, for cyathostomin research and highlight necessity for method standardization and raw data accessibility for optimal post-factum comparisons.
Digital Object Identifier (DOI)
Bellaw, Jennifer L. and Nielsen, Martin K., "Meta-Analysis of Cyathostomin Species-Specific Prevalence and Relative Abundance in Domestic Horses from 1975-2020: Emphasis on Geographical Region and Specimen Collection Method" (2020). Maxwell H. Gluck Equine Research Center Faculty Publications. 46.
Additional file 1: Table S1. Publication and dataset demographics.
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Additional file 2: Table S2. Pairwise comparisons of species prevalence (%) by region for 35 cyathostomin species within seven regions across 38 publications, 49 datasets, and 1592 hosts.
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Additional file 3: Table S3. Pairwise comparisons of species relative abundance (%) by region for 35 Cyathostominae species within five regions across 29 publications, 35 datasets, and 1217 equine hosts examined.
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Additional file 4: Table S4. Pairwise comparisons of species prevalence (%) by specimen collection method for 35 cyathostomin species for three methods across 49 datasets.
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Additional file 5: Table S5. Pairwise comparisons of species relative abundance (%) by specimen collection method for 35 cyathostomin species for three methods across 35 datasets.
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Additional file 6: Dataset S1. Complete data set from this study.
Published in Parasites & Vectors, v. 13, issue 1, article no. 509.
© The Author(s) 2020
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