miRNAs Regulated Overexpression of Ryanodine Receptor Is Involved in Chlorantraniliprole Resistance in Plutella xylostella (L.)

Authors

Xiuxia Li, China Agricultural University, China
Lei Guo, China Agricultural University, China
Xuguo Zhou, University of KentuckyFollow
Xiwu Gao, China Agricultural University, China
Pei Liang, China Agricultural University, China

Abstract

The amino acid mutations in ryanodine receptor (RyR) and elevated activity of detoxification enzymes have been associated with the diamide insecticide resistance in the diamondback moth, Plutella xylostella (L.). The up-regulation of P. xylostella RyR mRNA (PxRyR) expression has also been reported in field populations of different graphical origin. However, whether the up-regulation of PxRyR is involved in diamide resistance remains unknown. In this paper, 2.28- to 4.14-fold higher expression of PxRyR was detected in five field collected resistant populations, compared to that in a susceptible population. The expression of PxRyR was up-regulated 5.0- and 7.2-fold, respectively, after P. xylostella was treated with LC₅₀ and LC₇₅ of chlorantraniliprole for 12 h. Suppression of PxRyR using RNA interference restored the toxicity of chlorantraniliprole against the fourth instar larvae from the resistant population. More importantly, the expression of PxRyR is regulated by two miRNAs, miR-7a and miR-8519. These findings provide an empirical evidence of the involvement of miRNAs in the regulation of insecticide resistance, and shed light on the novel targets for the sustainable management of this devastating insect pest.

Document Type

Article

Publication Date

9-15-2015

Notes/Citation Information

Published Scientific Reports, v. 5, article 14095, p. 1-9.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Digital Object Identifier (DOI)

http://dx.doi.org/10.1038/srep14095

Funding Information

This work was supported by the National Basic Research Programme of China (2012CB114103), and the National Natural Science Foundation of China (31171873 and 31371956).

Repository Citation

Li, Xiuxia; Guo, Lei; Zhou, Xuguo; Gao, Xiwu; and Liang, Pei, "miRNAs Regulated Overexpression of Ryanodine Receptor Is Involved in Chlorantraniliprole Resistance in Plutella xylostella (L.)" (2015). Entomology Faculty Publications. 75.
https://uknowledge.uky.edu/entomology_facpub/75