Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Authors

Hao Yue, Hunan University, China
Li-Ping Huang, Hunan University, China
Ding-Li-Hui Lu, Hunan University, China
Zhan-Hong Zhang, Hunan Academy of Agricultural Sciences, China
Zhuo Zhang, Hunan Academy of Agricultural Sciences, China
De-Yong Zhang, Hunan Academy of Agricultural Sciences, China
Li-Min Zheng, Hunan Academy of Agricultural Sciences, China
Yang Gao, Hunan Academy of Agricultural Sciences, China
Xin-Qiu Tan, Hunan Academy of Agricultural Sciences, China
Xuguo Zhou, University of KentuckyFollow
Xiao-Bin Shi, Hunan University, China
Yong Liu, Hunan University, China

Abstract

Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.

Document Type

Article

Publication Date

6-22-2021

Notes/Citation Information

Published in Frontiers in Microbiology, v. 12, article 693574.

© 2021 Yue, Huang, Lu, Zhang, Zhang, Zhang, Zheng, Gao, Tan, Zhou, Shi and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Digital Object Identifier (DOI)

https://doi.org/10.3389/fmicb.2021.693574

Funding Information

This study was supported by Hunan Natural Science Foundation (2019JJ30014), the National Natural Science Foundation of China (31872932, 32030088, 31972242, and 32072383), and the Agriculture Research System of China (CARS-23-D-02).

Related Content

The RNA-seq reads have been submitted to the SRA at NCBI under the accession PRJNA699095.

Repository Citation

Yue, Hao; Huang, Li-Ping; Lu, Ding-Li-Hui; Zhang, Zhan-Hong; Zhang, Zhuo; Zhang, De-Yong; Zheng, Li-Min; Gao, Yang; Tan, Xin-Qiu; Zhou, Xuguo; Shi, Xiao-Bin; and Liu, Yong, "Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus" (2021). Entomology Faculty Publications. 221.
https://uknowledge.uky.edu/entomology_facpub/221