Abstract
Full length open reading frame of pyrethroid detoxification gene, Est3385, contains 963 nucleotides. This gene was identified and cloned based on the genome sequence of Rhodopseudomonas palustris PSB-S available at the GneBank. The predicted amino acid sequence of Est3385 shared moderate identities (30–46%) with the known homologous esterases. Phylogenetic analysis revealed that Est3385 was a member in the esterase family I. Recombinant Est3385 was heterologous expressed in E. coli, purified and characterized for its substrate specificity, kinetics and stability under various conditions. The optimal temperature and pH for Est3385 were 35 °C and 6.0, respectively. This enzyme could detoxify various pyrethroid pesticides and degrade the optimal substrate fenpropathrin with a Km and Vmax value of 0.734 ± 0.013 mmol·l−1 and 0.918 ± 0.025 U·µg−1, respectively. No cofactor was found to affect Est3385 activity but substantial reduction of enzymatic activity was observed when metal ions were applied. Taken together, a new pyrethroid degradation esterase was identified and characterized. Modification of Est3385 with protein engineering toolsets should enhance its potential for field application to reduce the pesticide residue from agroecosystems.
Document Type
Article
Publication Date
5-9-2018
Digital Object Identifier (DOI)
https://doi.org/10.1038/s41598-018-25734-9
Funding Information
This work was supported by the National Key R&D Program of China (2017YFD0800702), the National Natural Science Foundation of China (31272067, 3140110978), the Agriculture Research System of China (CARS-25-B-05), Hunan Science and technology project (2017JC44) and the Hunan Talent Project (2016RS2019).
Related Content
Electronic supplementary material: dataset 1
Repository Citation
Luo, Xiangwen; Zhang, Deyong; Zhou, Xuguo; Du, Jiao; Zhang, Songbai; and Liu, Yong, "Cloning and Characterization of a Pyrethroid Pesticide Decomposing Esterase Gene, Est3385, from Rhodopseudomonas palustris PSB-S" (2018). Entomology Faculty Publications. 151.
https://uknowledge.uky.edu/entomology_facpub/151
dataset 1
Included in
Agronomy and Crop Sciences Commons, Entomology Commons, Environmental Health Commons, Environmental Microbiology and Microbial Ecology Commons, Food Biotechnology Commons, Molecular Biology Commons, Other Ecology and Evolutionary Biology Commons
Notes/Citation Information
Published in Scientific Reports, v. 8, 7384, p. 1-8.
© The Author(s) 2018
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