MicroRNAs (miRNAs) are short single-stranded non-coding RNAs that regulate gene expression, particularly during development. In this study, 345 miRNAs were identified from the English green aphid, Sitobion avenae (F.), of which 168 were conserved and 177 were S. avenae-specific. Quantitative comparison of miRNA expression levels indicated that 16 and 12 miRNAs were significantly up-regulated in winged and wingless S. avenae small RNA libraries, respectively. Differential expression of these miRNAs was confirmed by real-time quantitative RT-PCR validation. The putative transcript targets for these candidate miRNAs were predicted based on sequences from a model species Drosophila melanogaster and four aphid species Acyrthosiphon pisum, Myzus persicae, Toxoptera citricida, and Aphis gosspii. Gene Ontology and KEGG pathway analyses shed light on the potential functions of these miRNAs in the regulation of genes involved in the metabolism, development and wing polyphenism of S. avenae.

Document Type


Publication Date


Notes/Citation Information

Published in Scientific Reports, v. 6, article no. 35668, p. 1-12.

© The Author(s) 2016

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Digital Object Identifier (DOI)


Funding Information

This research was supported by the National Natural Science Foundation of China (Award number: 31301659), National “Twelfth Five-Year” Plan for Science & Technology Support Development Program of China (Award number: 2012BAD19B04), China Agriculture Research System (Award number: CARS-3).

Related Content

The information reported in this paper (No. 16-08-098) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.

srep35668-s1.pdf (1116 kB)
Supplementary Information

srep35668-s2.xls (116 kB)
Supplementary Table S1

srep35668-s3.xls (114 kB)
Supplementary Table S2

srep35668-s4.xls (8064 kB)
Supplementary Table S3

srep35668-s5.xls (3500 kB)
Supplementary Table S4

srep35668-s6.xls (266 kB)
Supplementary Table S5

srep35668-s7.xls (19 kB)
Supplementary Table S6

Included in

Entomology Commons