Comparative Profiling of MicroRNAs in the Winged and Wingless English Grain Aphid, Sitobion avenae (F.) (Homoptera: Aphididae)

Authors

Xiangrui Li, Chinese Academy of Agricultural Sciences, China
Fangmei Zhang, Chinese Academy of Agricultural Sciences, China
Brad Coates, USDA-ARS
Yunhui Zhang, Chinese Academy of Agricultural Sciences, China
Xuguo Zhou, University of KentuckyFollow
Dengfa Cheng, Chinese Academy of Agricultural Sciences, China

Abstract

MicroRNAs (miRNAs) are short single-stranded non-coding RNAs that regulate gene expression, particularly during development. In this study, 345 miRNAs were identified from the English green aphid, Sitobion avenae (F.), of which 168 were conserved and 177 were S. avenae-specific. Quantitative comparison of miRNA expression levels indicated that 16 and 12 miRNAs were significantly up-regulated in winged and wingless S. avenae small RNA libraries, respectively. Differential expression of these miRNAs was confirmed by real-time quantitative RT-PCR validation. The putative transcript targets for these candidate miRNAs were predicted based on sequences from a model species Drosophila melanogaster and four aphid species Acyrthosiphon pisum, Myzus persicae, Toxoptera citricida, and Aphis gosspii. Gene Ontology and KEGG pathway analyses shed light on the potential functions of these miRNAs in the regulation of genes involved in the metabolism, development and wing polyphenism of S. avenae.

Document Type

Article

Publication Date

10-20-2016

Notes/Citation Information

Published in Scientific Reports, v. 6, article no. 35668, p. 1-12.

© The Author(s) 2016

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Digital Object Identifier (DOI)

https://doi.org/10.1038/srep35668

Funding Information

This research was supported by the National Natural Science Foundation of China (Award number: 31301659), National “Twelfth Five-Year” Plan for Science & Technology Support Development Program of China (Award number: 2012BAD19B04), China Agriculture Research System (Award number: CARS-3).

Related Content

The information reported in this paper (No. 16-08-098) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.

Repository Citation

Li, Xiangrui; Zhang, Fangmei; Coates, Brad; Zhang, Yunhui; Zhou, Xuguo; and Cheng, Dengfa, "Comparative Profiling of MicroRNAs in the Winged and Wingless English Grain Aphid, Sitobion avenae (F.) (Homoptera: Aphididae)" (2016). Entomology Faculty Publications. 112.
https://uknowledge.uky.edu/entomology_facpub/112