Author ORCID Identifier

Date Available


Year of Publication


Degree Name

Master of Science (MS)

Document Type

Master's Thesis


Arts and Sciences



First Advisor

Dr. Anne-Frances Miller


Flavoproteins have long been explored for their ubiquity among a number of metabolic and energetic reactions. The flavin cofactor has the inherent benefit of distinct spectral changes associated with redox transitions; however, the double-edged sword is incurred as these distinct signatures overlap and take up much of the UV-vis spectral range. Therefore, it is crucial to create a method to demarcate the expressed redox transitions for studying these systems. The first portion of these studies discusses the creation of a program that deduces spectra for redox transitions in a single-flavin containing model protein: flavodoxin. The latter portions discuss the application of the program in a much more challenging diflavin system (Rhodopseudomonas palustris ETF) for a variety of experiments, including separating spectral contributions from individual flavins and studying an intriguing feature, possibly associated with a conformational change. Together, these demonstrate the program’s ability to increase the insight obtainable from a variety of experiments.

Digital Object Identifier (DOI)