Author ORCID Identifier
Date Available
9-4-2021
Year of Publication
2020
Degree Name
Master of Science (MS)
Document Type
Master's Thesis
College
Arts and Sciences
Department/School/Program
Chemistry
First Advisor
Dr. Anne-Frances Miller
Abstract
Flavoproteins have long been explored for their ubiquity among a number of metabolic and energetic reactions. The flavin cofactor has the inherent benefit of distinct spectral changes associated with redox transitions; however, the double-edged sword is incurred as these distinct signatures overlap and take up much of the UV-vis spectral range. Therefore, it is crucial to create a method to demarcate the expressed redox transitions for studying these systems. The first portion of these studies discusses the creation of a program that deduces spectra for redox transitions in a single-flavin containing model protein: flavodoxin. The latter portions discuss the application of the program in a much more challenging diflavin system (Rhodopseudomonas palustris ETF) for a variety of experiments, including separating spectral contributions from individual flavins and studying an intriguing feature, possibly associated with a conformational change. Together, these demonstrate the program’s ability to increase the insight obtainable from a variety of experiments.
Digital Object Identifier (DOI)
https://doi.org/10.13023/etd.2020.400
Recommended Citation
Bell, Dallas Michael, "Deconvolution Tools for Extracting Insight from Challenging Two-Flavin Systems" (2020). Theses and Dissertations--Chemistry. 132.
https://uknowledge.uky.edu/chemistry_etds/132
Included in
Biochemistry Commons, Biophysics Commons, Data Science Commons
