Abstract

The purpose of the report is to describe dissection techniques for preparing the crayfish hindgut and to demonstrate how to make physiological recordings with a force transducer to monitor the strength of contraction. In addition, we demonstrate how to visually monitor peristaltic activity, which can be used as a bioassay for various peptides, biogenic amines and neurotransmitters. This preparation is amenable to student laboratories in physiology and for demonstrating pharmacological concepts to students. This preparation has been in use for over 100 years, and it still offers much as a model for investigating the generation and regulation of peristaltic rhythms and for describing the mechanisms underlying their modulation. The pharmacological assays and receptor sub-typing that were started over 50 years ago on the hindgut still contribute to research today. This robust preparation is well suited to training students in physiology and pharmacology.

Document Type

Article

Publication Date

1-18-2011

Notes/Citation Information

Published in Journal of Visualized Experiments, v. 47, e2324, p. 1-6.

Copyright © 2011 Creative Commons Attribution-NonCommercial License.

Digital Object Identifier (DOI)

http://dx.doi.org/10.3791/2324

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