Abstract
The regeneration blastema which forms following amputation of the mouse digit tip is composed of undifferentiated cells bound together by an organized network of fibers. A monoclonal antibody (ER‐TR7) that identifies extracellular matrix (ECM) fibers produced by fibroblast reticular cells during lymphoid organogenesis was used to characterize the ECM of the digit, the blastema, and the regenerate. Digit fibroblast reticular cells produce an ER‐TR7+ ECM network associated with different tissues and represent a subset of loose connective tissue fibroblasts. During blastema formation there is an upregulation of matrix production that returns to its pre‐existing level and anatomical pattern in the endpoint regenerate. Co‐localization studies demonstrate a strong spatial correlation between the ER‐TR7 antigen and collagen type III (COL3) in histological sections. ER‐TR7 and COL3 are co‐induced in cultured digit fibroblasts following treatment with tumor necrosis factor alpha and a lymphotoxin beta receptor agonist. These results provide an initial characterization of the ECM during digit regeneration and identify a subpopulation of fibroblasts involved in producing the blastema provisional matrix that is remodeled during the regeneration response.
Document Type
Article
Publication Date
4-2017
Digital Object Identifier (DOI)
https://doi.org/10.1002/reg2.75
Funding Information
This work was supported by W911NF‐06‐1‐0161 from DARPA, awarded to KM as lead PI and LM as co‐PI, and internal funds from the Morphology and Imaging Core of the LSU Health School of Medicine.
Related Content
Additional Supporting Information may be found online in the supporting information tab for this article.
Repository Citation
Marrero, Luis; Simkin, Jennifer; Sammarco, Mimi; and Muneoka, Ken, "Fibroblast Reticular Cells Engineer a Blastema Extracellular Network During Digit Tip Regeneration in Mice" (2017). Biology Faculty Publications. 132.
https://uknowledge.uky.edu/biology_facpub/132
Fig. S1.
reg275-sup-0002-FigureS1.pdf (135 kB)
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reg275-sup-0003-FigureS1.pdf (2379 kB)
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reg275-sup-0004-tables1.pdf (58 kB)
Table S1.
Notes/Citation Information
Published in Regeneration, v. 4, issue 2, p. 69-84.
© 2017 The Authors.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.