Date Available
7-17-2016
Year of Publication
2016
Document Type
Master's Thesis
Degree Name
Master of Science in Biosystems and Agricultural Engineering (MSBiosyAgE)
College
Agriculture; Engineering
Department/School/Program
Biosystems and Agricultural Engineering
Advisor
Dr. Sue E. Nokes
Abstract
This project aimed investigated cellulase in-situ production for large-scale on-farm production of lignocellulosic biofuel. Cellulase activity and glucose released by T. reesei with corn stover and wheat bran as co-substrates for solid state cultivation (SSC) were examined. Co-cultivation has previously increased T. reesei cellulase, but corn stover and wheat bran have not been co-cultivated (Dhillon, Oberoi et al. 2011). This work compared cellulase activity and glucose concentration of corn stover co-cultivated with 0-40% wheat bran in high solids. Samples with at least 20% wheat bran exhibited increased cellulase activity. However, the average glucose concentration without wheat bran was 3.29 g/L compared to 16.7 g/L with wheat bran.
Glucose released by T. reesei on pretreated corn stover with 0-40% wheat bran was compared at the optimal temperatures for fungal growth and for cellulase activity after SSC. Previous research has rarely used cellulase from SSC to hydrolyze lignocellulose. Following SSC of T. reesei at 30°C for seven days, samples were warmed to 50°C for five days. Glucose concentration increased to 12.1 and 32.7 g/L for samples with and without wheat bran. This strategy could reduce lignocellulosic fuel production costs by eliminating need for commercial cellulase and is promising for efficient cellulose hydrolysis.
Digital Object Identifier (DOI)
http://dx.doi.org/10.13023/ETD.2016.372
Recommended Citation
Empson, Danielle, "INDUCTION OF CELLULASE IN HIGH SOLIDS CULTIVATION OF TRICHODERMA REESEI FOR ENHANCED ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSE" (2016). Theses and Dissertations--Biosystems and Agricultural Engineering. 46.
https://uknowledge.uky.edu/bae_etds/46
