US Patent Number
4999283
Publication Date
3-12-1991
Abstract
A method for separating male and female determining spermatozoa includes the initial step of exposing freshly ejaculated spermatozoa in a substantially protein free diluent to an excess concentration of a monoclonal antibody directed against H-Y antigen that binds substantially exclusively with male determining spermatozoa. The method continues with the suspending of the exposed spermatozoa together with a conjugate of (1) an immunoglobulin G antibody that binds substantially exclusively to the monoclonal antibody and (2) an immunoabsorbant substrate in a substantially protein free diluent. This forms a conjugate/spermatozoa preparation. The method concludes with the recovering of the separated male and female determining spermatozoa.
Assignees
University of Kentucky Research Foundation, Lexington, Ky.
Application Number
396,738
Filing Date
08/18/1989
Recommended Citation
Zavos, Panayiotis M. and Dawson, Karl A., "Method for X and Y Spermatozoa Separation" (1991). Animal and Food Sciences Faculty Patents. 10.
https://uknowledge.uky.edu/animalsci_patents/10
