Author ORCID Identifier
Date Available
12-5-2017
Year of Publication
2017
Document Type
Master's Thesis
Degree Name
Master of Science (MS)
College
Agriculture, Food and Environment
Department/School/Program
Animal and Food Sciences
Advisor
Dr. James L. Klotz
Abstract
Prior work using a contractility bioassay determined that the serotonin (5-HT) receptor subtype 5-HT2A is present in bovine lateral saphenous veins and plays a role in ergot alkaloid-induced vascular contraction in steers grazing endophyte-infected (Epichloë coenophiala) tall fescue (Lolium arundinaceum). A study was conducted to determine what 5-HT receptors are involved in vasoconstriction of bovine gut vasculature. The findings of this study indicate that 5-HT2A is present and may play a role in ergot alkaloid induced vasoconstriction. A second study was conducted to determine if ergot alkaloids were transported in the small intestine. The active transporter, peptide transporter 1 (PepT1), was evaluated for its role in the transport of various concentrations of ergot alkaloids across Caco-2 cell monolayers. Results indicate that CEPH, ERT, EXT, and LSA do move across Caco-2 cell monolayers, but appear to utilize PepT1 at larger concentrations. Overall, the demonstrated presence of 5-HT2A receptors in the bovine gut vasculature established a potential for vascular interference by ergot alkaloids entering the bloodstream through transepithelial absorption.
Digital Object Identifier (DOI)
https://doi.org/10.13023/ETD.2017.456
Recommended Citation
Snider, Miriam A., "ASSESSMENT OF BOVINE VASCULAR SEROTONIN RECEPTOR POPULATIONS AND TRANSPORT OF ERGOT ALKALOIDS IN THE SMALL INTESTINE" (2017). Theses and Dissertations--Animal and Food Sciences. 79.
https://uknowledge.uky.edu/animalsci_etds/79
