Date Available
12-9-2015
Year of Publication
2015
Document Type
Master's Thesis
Degree Name
Master of Science (MS)
College
Agriculture, Food and Environment
Department/School/Program
Animal and Food Sciences
Advisor
Dr. Eric S. Vanzant
Abstract
Acute phase responses (APR) and circadian rhythm of body temperature (CRT) changes to a minor, chronic stressor (endophyte) and major, acute stressor, lipopolysaccharide (LPS) were evaluated in two experiments (E1:22 heifers; E2:24 heifers). Vaginal (E1 and E2) and rectal (E2) temperature probes recorded body temperature. Temperature was analyzed by cosinor analysis in each of 4 Phases: pre-endophyte (P1), endophyte exposure, pre-LPS (P2), post-LPS spike 1 (P3), post-LPS spike 2 (P4). During P1 of E1, amplitude (P=0.05), but not mean temperatures (P>0.10), differed across days (P=0.05). Endophyte affected E2 vaginal temperature amplitudes in P2 (P=0.07) and P4 (P=0.08) without influencing mean temperatures. Significant treatment*weaning exit velocity (wEV) interactions occurred for E2 mean rectal temperatures during P1-P3 (P≤0.10). During E1, post LPS, endophyte depressed glucose (P=0.05) and increased creatinine (P<0.01) and decreased TNF-α (P<0.01) at 24h. During E2, post-LPS, IFN-ɤ decreased (P=0.02) with increasing wEV. Endophyte increased serum BUN concentrations with slow wEV and increased BUN with fast wEV (P=0.04) post-LPS in E2. Endophyte increased NEFA (P<0.01) and IL-6 (P=0.02) from 1-8 hours post-LPS during E2. Results demonstrate modulation of CRT and APR by both endophyte and wEV, and demonstrate effectiveness of cosinor analysis in detecting CRT responses.
Recommended Citation
Altman, Alexander W., "Impact of Endophyte-Infected Tall Fescue Seed on the Acute Phase and Metabolic Responses of Cattle During an Immunological Challenge" (2015). Theses and Dissertations--Animal and Food Sciences. 56.
https://uknowledge.uky.edu/animalsci_etds/56
