Description
Tillering is an important trait of bunch grass that affects biomass and seed yield. Psathyrostachys juncea is a typical perennial bunch grass, and unraveling the regulatory mechanisms of tillering in P. juncea could be helpful to improve the yield of perennial gramineous forages. Hence, we selected the tiller node of P. juncea for transcriptome sequencing to determine the differentially expressed genes (DEG) between high and low tillering materials. The metabolic pathway was studied，candidate genes were screened, and reference genes stability were evaluated. The results showed that approximately 5466 DEGs were identified between two P. juncea genotypes that significantly differed in tiller number. Pathway enrichment analysis indicated that DEGs related to the biosynthesis of three classes of phytohormones, i.e., strigolactones (SLs), auxin (IAA), and cytokinin (CTK), as well as “nitrogen metabolism” and “biosynthesis of lignin” dominated the differences between the dense and sparse tillering genotypes. Meanwhile, the reference gene Actin1, having with the best stability, was screened from twelve highest expression level genes and was used in verification of ten tillering candidate genes. The candidate genes revealed in our research are involved in the regulation of tillering in perennial grasses and are available for new breeding resources establishment for high-yield perennial grasses.
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Analysis of Controlling Genes for Tiller Growth of Psathyrostachys juncea Based on Transcriptome Sequencing Technology
Tillering is an important trait of bunch grass that affects biomass and seed yield. Psathyrostachys juncea is a typical perennial bunch grass, and unraveling the regulatory mechanisms of tillering in P. juncea could be helpful to improve the yield of perennial gramineous forages. Hence, we selected the tiller node of P. juncea for transcriptome sequencing to determine the differentially expressed genes (DEG) between high and low tillering materials. The metabolic pathway was studied，candidate genes were screened, and reference genes stability were evaluated. The results showed that approximately 5466 DEGs were identified between two P. juncea genotypes that significantly differed in tiller number. Pathway enrichment analysis indicated that DEGs related to the biosynthesis of three classes of phytohormones, i.e., strigolactones (SLs), auxin (IAA), and cytokinin (CTK), as well as “nitrogen metabolism” and “biosynthesis of lignin” dominated the differences between the dense and sparse tillering genotypes. Meanwhile, the reference gene Actin1, having with the best stability, was screened from twelve highest expression level genes and was used in verification of ten tillering candidate genes. The candidate genes revealed in our research are involved in the regulation of tillering in perennial grasses and are available for new breeding resources establishment for high-yield perennial grasses.
