Description
The objective of this study was to evaluate the chemical composition and microbial diversity of the silage of forage peanut harvested at 40 and 80 days of regrowth and treated with microbial inoculants. The inoculants evaluated were commercial inoculant (CI) Sil-All 4×4 (Lallemand®, Brazil); the AV14.17, Lactiplantibacillus pentosus strain (ISO) isolated from alfalfa silage; and control (CTRL), without inoculant. A total of 500 g of forage was ensiled in nylon-polyethylene bags (25.40 × 35.56 cm). After 60 days of fermentation, the bags were opened and silages were analyzed for dry matter (DM), mineral matter (MM), organic matter (OM), crude protein (CP), neutral detergent fiber corrected for ash and protein (NDFap), acid detergent fiber (ADF), acid detergent insoluble nitrogen (ADIN), and lignin (LIG). After obtaining the aqueous extract of the silage, microbial DNA was extracted to evaluate the microbial diversity, through amplification of the 16S rDNA gene. Data were analyzed in a completely randomized block design, in a 2 × 3 factorial arrangement [regrowth age (R) × inoculant (I)] with four replicates, adopting the significance level of P≤ 0.05. Tukey’s test was used to perform paired comparisons using the glht-multicomp function. There was variation (P≤ 0.05) in the relative abundance of bacterial community of forage peanut silage. There was no effect (P> 0.05) of the R × I interaction on the evaluated traits. However, an effect (P≤ 0.05) of I and R was observed on the ADF content, and of R on MM, OM, and ADIN contents. After ensiling, there was a decrease in bacterial genera with a predominance of Enterobacter and Lactiplantibacillus at both regrowth ages. The ISO-treated silages showed higher relative abundance for the genus Lactiplantibacillus. Silage of forage peanut is recommend at both regrowth ages (40 and 80 days) associated with wilting and microbial inoculants to promote improvements in the fermentative characteristics of the silage.
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Chemical Composition and Microbial Diversity of the Silage of Forage Peanut Harvested at Two Regrowth Ages and Treated With Microbial Inoculants
The objective of this study was to evaluate the chemical composition and microbial diversity of the silage of forage peanut harvested at 40 and 80 days of regrowth and treated with microbial inoculants. The inoculants evaluated were commercial inoculant (CI) Sil-All 4×4 (Lallemand®, Brazil); the AV14.17, Lactiplantibacillus pentosus strain (ISO) isolated from alfalfa silage; and control (CTRL), without inoculant. A total of 500 g of forage was ensiled in nylon-polyethylene bags (25.40 × 35.56 cm). After 60 days of fermentation, the bags were opened and silages were analyzed for dry matter (DM), mineral matter (MM), organic matter (OM), crude protein (CP), neutral detergent fiber corrected for ash and protein (NDFap), acid detergent fiber (ADF), acid detergent insoluble nitrogen (ADIN), and lignin (LIG). After obtaining the aqueous extract of the silage, microbial DNA was extracted to evaluate the microbial diversity, through amplification of the 16S rDNA gene. Data were analyzed in a completely randomized block design, in a 2 × 3 factorial arrangement [regrowth age (R) × inoculant (I)] with four replicates, adopting the significance level of P≤ 0.05. Tukey’s test was used to perform paired comparisons using the glht-multicomp function. There was variation (P≤ 0.05) in the relative abundance of bacterial community of forage peanut silage. There was no effect (P> 0.05) of the R × I interaction on the evaluated traits. However, an effect (P≤ 0.05) of I and R was observed on the ADF content, and of R on MM, OM, and ADIN contents. After ensiling, there was a decrease in bacterial genera with a predominance of Enterobacter and Lactiplantibacillus at both regrowth ages. The ISO-treated silages showed higher relative abundance for the genus Lactiplantibacillus. Silage of forage peanut is recommend at both regrowth ages (40 and 80 days) associated with wilting and microbial inoculants to promote improvements in the fermentative characteristics of the silage.
