Description
Temperature is an important factor affecting ensilage. In cold regions, low temperature could be an adverse environmental factor during ensiling. However, little research has focused on improving silage quality at low temperatures. This study aimed to examine two lactic acid bacteria (LAB) strains (LCG9 and TG1) isolated from the Tibetan Plateau, and evaluate their effects on the silage quality of Italian ryegrass (Lolium multiflorum Lam.) at three temperatures (10° C, 15° C and 25° C). The isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated by morphological, physiological and biochemical tests. Strains G, LCG9, TG1 and their combination of LCG9+TG1 were added to Italian ryegrass for ensiling 90 days at various temperatures. All the isolates could grow normally at 5-20°C, pH 3.5-7.0 and NaCl (3.0%, 6.5%). Strains LCG9 and TG1 were identified as Pediococcus pentosaceus and Lactobacillus plantarum by sequencing 16S rDNA, respectively. Compared to the corresponding controls, all the inoculants improved the silage quality of Italian ryegrass at different temperatures, indicated by significantly (P3- N) contents and undesirable microorganism counts. At 10°C and 15°C, strains TG1 and G performed better than other inoculants, indicated by significantly (P3-N contents. Strains TG1 and G are recommended as starter culture for Italian ryegrass silage at low temperatures.
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Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Characteristics of Lactic Acid Bacteria Strains Isolated From Tibetan Plateau and Their Effects on Silage Quality of Italian Ryegrass (Lolium Multiflorum Lam.) At Low Temperature
Temperature is an important factor affecting ensilage. In cold regions, low temperature could be an adverse environmental factor during ensiling. However, little research has focused on improving silage quality at low temperatures. This study aimed to examine two lactic acid bacteria (LAB) strains (LCG9 and TG1) isolated from the Tibetan Plateau, and evaluate their effects on the silage quality of Italian ryegrass (Lolium multiflorum Lam.) at three temperatures (10° C, 15° C and 25° C). The isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated by morphological, physiological and biochemical tests. Strains G, LCG9, TG1 and their combination of LCG9+TG1 were added to Italian ryegrass for ensiling 90 days at various temperatures. All the isolates could grow normally at 5-20°C, pH 3.5-7.0 and NaCl (3.0%, 6.5%). Strains LCG9 and TG1 were identified as Pediococcus pentosaceus and Lactobacillus plantarum by sequencing 16S rDNA, respectively. Compared to the corresponding controls, all the inoculants improved the silage quality of Italian ryegrass at different temperatures, indicated by significantly (P3- N) contents and undesirable microorganism counts. At 10°C and 15°C, strains TG1 and G performed better than other inoculants, indicated by significantly (P3-N contents. Strains TG1 and G are recommended as starter culture for Italian ryegrass silage at low temperatures.
