Publication Date
1997
Description
The proanthocyanidin (butanol-HCl) assay was used to measure condensed tannin (CT) in extracts from Leucaena spp. at the University of Queensland. Inconsistent results were found to be caused by the iron catalyst in the butanol/HCl reagent and the presence of ascorbic acid in the sample matrix which enhanced anthocyanidin development. In addition, preparation of sample extracts by back extraction with organic solvents reduced CT recoveries. An accurate and rapid technique was developed that measures CT directly in 70% aqueous acetone 0.1% (w/v) sodium metabisulphite plant extracts.
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
A Revised Method of Condensed Tannin Analysis in Leucaena spp.
The proanthocyanidin (butanol-HCl) assay was used to measure condensed tannin (CT) in extracts from Leucaena spp. at the University of Queensland. Inconsistent results were found to be caused by the iron catalyst in the butanol/HCl reagent and the presence of ascorbic acid in the sample matrix which enhanced anthocyanidin development. In addition, preparation of sample extracts by back extraction with organic solvents reduced CT recoveries. An accurate and rapid technique was developed that measures CT directly in 70% aqueous acetone 0.1% (w/v) sodium metabisulphite plant extracts.
