Description
Interspecific hybridization between white clover (Trifolium repens L.) and ball clover (T.nigrescens) and Caucasian clover (T.ambiguum) is a means of improving the reproductive growth and persistency of T.repens. F1 hybrids between T.repens and T.nigrescens were produced without ovule culture and confirmed as true hybrids by chromosome counts, isoenzyme analysis and leaf markers. Their growth habit was generally intermediate between the two parents. After backcrossing to T.repens the BC1 and BC2 hybrids had more of the characteristics associated with T.repens though they retained some of the greater reproductive growth of T.nigrescens. Hybrids between T.repens and T.ambiguum were produced using ovule culture. Two F1 seedlings were produced with a morphology intermediate to the two parents. 35 BC1 seedlings were produced with a similar morphology to the F1 but with less of the T.ambiguum growth habit. The BC2 population showed greater variability in the expression of characters from Caucasian clover and contained only 10% of plants with the combined stoloniferous and rhizomatous habit. The implications for improving the growth of T.repens by interspecific hybridization are discussed.
Included in
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Introduction of Vegetative and Reproductive Characters into Trifolium Repens by Interspecific Hybridisation
Interspecific hybridization between white clover (Trifolium repens L.) and ball clover (T.nigrescens) and Caucasian clover (T.ambiguum) is a means of improving the reproductive growth and persistency of T.repens. F1 hybrids between T.repens and T.nigrescens were produced without ovule culture and confirmed as true hybrids by chromosome counts, isoenzyme analysis and leaf markers. Their growth habit was generally intermediate between the two parents. After backcrossing to T.repens the BC1 and BC2 hybrids had more of the characteristics associated with T.repens though they retained some of the greater reproductive growth of T.nigrescens. Hybrids between T.repens and T.ambiguum were produced using ovule culture. Two F1 seedlings were produced with a morphology intermediate to the two parents. 35 BC1 seedlings were produced with a similar morphology to the F1 but with less of the T.ambiguum growth habit. The BC2 population showed greater variability in the expression of characters from Caucasian clover and contained only 10% of plants with the combined stoloniferous and rhizomatous habit. The implications for improving the growth of T.repens by interspecific hybridization are discussed.
