Publication Date

1993

Description

Three cellobiohydrolases were highly puriried from the rumen fungus Neocallimastix patriciarun. Two of these enzymes were liberated from insoluble material by extraction with the non-ionic detergent Triton X-114, requiring the presence of detergent or dissociating agent throughout their purification. The native cellobiohydrolase is probably membrane associated.

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Cellobiohydrolase (CBH) from Neocallimastix patriciarum: A Membrane Associated Complex?

Three cellobiohydrolases were highly puriried from the rumen fungus Neocallimastix patriciarun. Two of these enzymes were liberated from insoluble material by extraction with the non-ionic detergent Triton X-114, requiring the presence of detergent or dissociating agent throughout their purification. The native cellobiohydrolase is probably membrane associated.