Abstract
BACKGROUND: Age-related neurodegenerative diseases share a number of important pathological features, such as accumulation of misfolded proteins as amyloid oligomers and fibrils. Recent evidence suggests that soluble amyloid oligomers and not the insoluble amyloid fibrils may represent the primary pathological species of protein aggregates.
RESULTS: We have produced several monoclonal antibodies that specifically recognize prefibrillar oligomers and do not recognize amyloid fibrils, monomer or natively folded proteins. Like the polyclonal antisera, the individual monoclonals recognize generic epitopes that do not depend on a specific linear amino acid sequence, but they display distinct preferences for different subsets of prefibrillar oligomers. Immunological analysis of a number of different prefibrillar Aβ oligomer preparations show that structural polymorphisms exist in Aβ prefibrillar oligomers that can be distinguished on the basis of their reactivity with monoclonal antibodies. Western blot analysis demonstrates that the conformers defined by the monoclonal antibodies have distinct size distributions, indicating that oligomer structure varies with size. The different conformational types of Aβ prefibrillar oligomers can serve as they serve as templates for monomer addition, indicating that they seed the conversion of Aβ monomer into more prefibrillar oligomers of the same type.
CONCLUSIONS: These results indicate that distinct structural variants or conformers of prefibrillar Aβ oligomers exist that are capable of seeding their own replication. These conformers may be analogous to different strains of prions.
Document Type
Article
Publication Date
12-13-2010
Digital Object Identifier (DOI)
http://dx.doi.org/10.1186/1750-1326-5-57
Repository Citation
Kayed, Rakez; Canto, Isabel; Breydo, Leonid; Rasool, Suhail; Lukacsovich, Tamas; Wu, Jessica; Albay, Ricardo; Pensalfini, Anna; Yeung, Stephen; Head, Elizabeth; Marsh, J. Lawrence; and Glabe, Charles, "Conformation dependent monoclonal antibodies distinguish different replicating strains or conformers of prefibrillar Aβ oligomers" (2010). Sanders-Brown Center on Aging Faculty Publications. 23.
https://uknowledge.uky.edu/sbcoa_facpub/23
Notes/Citation Information
Published in Molecular Neurodegeneration, v. 5, 57.
© 2010 Kayed et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.