Disrupted Calcium Dynamics in Reactive Astrocytes Occur with End Feet–Arteriole Decoupling in an Amyloid Mouse Model of Alzheimer’s Disease

Authors

• Blaine Weiss, University of KentuckyFollow
• John C. Gant, University of KentuckyFollow
• Ruei-Lung Lin, University of KentuckyFollow
• Jenna L. Gollihue, University of KentuckyFollow
• Colin B. Rogers, University of Kentucky
• Susan D. Kraner, University of KentuckyFollow
• Edmund B. Rucker, University of KentuckyFollow
• Yuriko Katsumata, University of KentuckyFollow
• Yang Jiang, University of KentuckyFollow
• Peter T. Nelson, University of KentuckyFollow
• Donna M. Wilcock, Stark Neuroscience Research Institute
• Pradoldej Sompol, University of KentuckyFollow
• Olivier Thibault, University of KentuckyFollow
• Christopher M. Norris, University of KentuckyFollow

Abstract

While cerebrovascular dysfunction and reactive astrocytosis are extensively characterized hallmarks of Alzheimer's disease (AD) and related dementias, the dynamic relationship between reactive astrocytes and cerebral vessels remains poorly understood. Here, we used jGCaMP8f and two-photon microscopy to investigate calcium signaling in multiple astrocyte subcompartments, concurrent with changes in cerebral arteriole activity, in fully awake 7- to 8-month-old male and female 5xFAD mice, a model for AD-like pathology, and wild-type (WT) littermates. In the absence of movement, spontaneous calcium transients in barrel cortex occurred more frequently in astrocyte somata, processes, and perivascular regions of 5xFAD mice. However, evoked arteriole dilations (in response to air puff stimulation of contralateral whiskers) and concurrent calcium transients across astrocyte compartments were reduced in 5xFAD mice relative to WTs. Synchronous activity within multicell astrocyte networks was also impaired in the 5xFAD group. Using a custom application to assess functional coupling between astrocyte end feet and immediately adjacent arteriole segments, we detected deficits in calcium response probability in 5xFAD mice. Moreover, end feet calcium transients following arteriole dilations exhibited a slower onset, reduced amplitude, and lacked relative proportionality to vasomotive activity compared with WTs. The results reveal nuanced alterations in 5xFAD reactive astrocytes highlighted by impaired signaling fidelity between astrocyte end feet and cerebral arterioles. The results have important implications for the mechanistic underpinnings of brain hypometabolism and the disruption of neurophysiologic communication found in AD and other neurodegenerative conditions.

Document Type

Article

Publication Date

2025

Notes/Citation Information

Copyright © 2025 the authors

Digital Object Identifier (DOI)

https://doi.org/10.1523/JNEUROSCI.0349-25.2025

Funding Information

This work was supported by National Institutes of Health (NIH)–National Institute on Aging Grants AG078116 to C.M.N., Y.K., Y.J., P.T.N., D.M.W., O.T., and P.S. and AG027297 C.M.N.; the Hazel Embry Research Trust; and the Sylvia Mansbach Endowment for Alzheimer’s Disease Research.

Repository Citation

Weiss, Blaine; Gant, John C.; Lin, Ruei-Lung; Gollihue, Jenna L.; Rogers, Colin B.; Kraner, Susan D.; Rucker, Edmund B.; Katsumata, Yuriko; Jiang, Yang; Nelson, Peter T.; Wilcock, Donna M.; Sompol, Pradoldej; Thibault, Olivier; and Norris, Christopher M., "Disrupted Calcium Dynamics in Reactive Astrocytes Occur with End Feet–Arteriole Decoupling in an Amyloid Mouse Model of Alzheimer’s Disease" (2025). Sanders-Brown Center on Aging Faculty Publications. 213.
https://uknowledge.uky.edu/sbcoa_facpub/213