Abstract
This protocol details the extraction of microsomes from frozen tissue in order to further examine the protein-protein interactions occurring within the endoplasmic reticulum. This protocol was adapted from Abisambra et al. (2013) with modifications made in order to optimize for subsequent use.
Document Type
Article
Publication Date
2-5-2014
Digital Object Identifier (DOI)
https://doi.org/10.21769/BioProtoc.1038
Funding Information
Financial support during the time of protocol development came from the Alzheimer’s Association NIRGD-12-242642, the Foundation for PSP/CBD and Related Brain Disorders (6144107400), and NIH/NIA ADC Pilot Grant from 5P30AG028383-08.
Repository Citation
Bodero, Maria and Abisambra, Jose Francisco, "Microsome Isolation from Tissue" (2014). Sanders-Brown Center on Aging Faculty Publications. 116.
https://uknowledge.uky.edu/sbcoa_facpub/116
Notes/Citation Information
Published in Bio-protocol, v. 4, issue 3, p. 1-3.
Copyright © 2014 The Authors; exclusive licensee Bio-protocol LLC.
The publisher has granted the permission for posting the article here.