Abstract
Moyamoya is a cerebrovascular disorder characterized by progressive stenosis of the intracranial internal carotid arteries. There are two forms: Disease and Syndrome, with each characterized by the sub-population it affects. Moyamoya syndrome (MMS) is more prominent in adults in their 20’s-40’s, and is often associated with autoimmune diseases. Currently, there are no surgical models for inducing moyamoya syndrome, so our aim was to develop a new animal model to study this relatively unknown cerebrovascular disease. Here, we demonstrate a new surgical technique termed internal carotid artery stenosis (ICAS), to mimic MMS using micro-coils on the proximal ICA. We tested for Moyamoya-like vasculopathies by fluorescently labelling the mouse cerebrovasculature with Di I for visualization and analysis of vessel diameter at the distal ICA and anastomoses on the cortical surface. Results show a significant narrowing of the distal ICA and anterior cerebral artery (ACA) in the Circle of Willis, as observed in humans. There is also a significant decrease in the number of anastomoses between the middle cerebral artery (MCA) and the ACA in the watershed region of the cortex. While further characterization is needed, this ICAS model can be applied to transgenic mice displaying co-morbidities as observed within the Moyamoya syndrome population, allowing a better understanding of the disease and development of novel treatments.
Document Type
Article
Publication Date
1-11-2018
Digital Object Identifier (DOI)
https://doi.org/10.1371/journal.pone.0191312
Funding Information
This work was supported by the National Institute of Health, Grant # 1 R01 NS089515-01 (www.nih.gov) to GJB.
Repository Citation
Roberts, Jill M.; Maniskas, Michael E.; Fraser, Justin F.; and Bix, Gregory J., "Internal Carotid Artery Stenosis: A Novel Surgical Model for Moyamoya Syndrome" (2018). Sanders-Brown Center on Aging Faculty Publications. 109.
https://uknowledge.uky.edu/sbcoa_facpub/109
Notes/Citation Information
Published in PLOS ONE, v. 13, no. 1, e0191312, p. 1-10.
© 2018 Roberts et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.