Shapes of edible plant organs vary dramatically among and within crop plants. To explain and ultimately employ this variation towards crop improvement, we determined the genetic, molecular and cellular bases of fruit shape diversity in tomato. Through positional cloning, protein interaction studies, and genome editing, we report that OVATE Family Proteins and TONNEAU1 Recruiting Motif proteins regulate cell division patterns in ovary development to alter final fruit shape. The physical interactions between the members of these two families are necessary for dynamic relocalization of the protein complexes to different cellular compartments when expressed in tobacco leaf cells. Together with data from other domesticated crops and model plant species, the protein interaction studies provide possible mechanistic insights into the regulation of morphological variation in plants and a framework that may apply to organ growth in all plant species.
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Funding for this research has been provided by National Science Foundation IOS 0922661, the Ohio State Tornado Recovery Fund OHOA0499, Ohio Agricultural Research and Development Center SEEDS grant 2014018, University of Georgia 1021RX070014, USDA AFRI National Institute of Food and Agriculture 2017-67013-26199 (EvdK). Ohio Agricultural Research and Development Center SEEDS grant 2014084 (SW). The Spanish Ministry of Economy and Competitiveness/FEDER grant AGL2015-64625-C2-2-R (AJM). The USDA AFRI National Institute of Food and Agriculture 2015-51181-24285 (YW). The USDA AFRI National Institute of Food and Agriculture 2014-67013-22434 (SHJ).
Raw sequence reads have been deposited into the DNA DataBank of Japan (DDBJ) under accessions SRP090032; SRP089970; SRA061767 for the mRNA seq data; and SRP127270 for the whole genome sequence data. The normalized expression data are also available at the Tomato Functional Genomics Database (TFGD; http://ted.bti.cornell.edu/) under the experiments D015 and D016. A Reporting Summary for this Article is available as a Supplementary Information file. Other methods can be found in the Supplementary Methods section in the Supplementary Information file.
Supplementary Information accompanies this paper at https://doi.org/10.1038/s41467-018-07216-8.
Wu, Shan; Zhang, Biyao; Keyhaninejad, Neda; Rodríguez, Gustavo R.; Kim, Hyun Jung; Chakrabarti, Manohar; Illa-Berenguer, Eudald; Taitano, Nathan K.; Gonzalo, M. J.; Díaz, Aurora; Pan, Yupeng; Leisner, Courtney P.; Halterman, Dennis; Buell, C. Robin; Weng, Yiqun; Jansky, Shelley H.; van Eck, Herman; Willemsen, Johan; Monforte, Antonio J; Meulia, Tea; and van der Knaap, Esther, "A Common Genetic Mechanism Underlies Morphological Diversity in Fruits and Other Plant Organs" (2018). Plant and Soil Sciences Faculty Publications. 118.
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Description of Additional Supplementary Files
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Supplementary Data 1
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Supplementary Data 2
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Supplementary Data 3
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Supplementary Data 4
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