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Abstract

Background: Most preclinical studies on glioblastoma (GBM) fail to provide translational utility in the clinic. Fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA) improves tumor resection, disease prognosis, and, thus, patient outcomes. Given the critical role of surgery in managing recurrent GBM, it is essential to incorporate surgical elements into preclinical models to accurately reflect clinical scenarios and enhance translational success. However, existing protocols for 5-ALA-guided resection in preclinical models are limited and often lack clinical relevance.

Methods: To address this gap, we developed a novel protocol for the 5-ALA-guided resection in two mouse GBM models: TRP-mCherry-FLuc and GL261 Red-FLuc.

Results: The resection of TRP-mCherry-FLuc tumors significantly extended survival and mitigated weight loss compared to controls. Similarly, GL261 Red-FLuc tumor resection increased survival, reduced body weight loss, and slowed tumor progression.

Conclusions: This study presents a clinically relevant protocol for 5-ALA-guided resection in preclinical GBM models, providing a platform for future research to integrate adjuvant therapies and enhance their potential translation into clinical practice.

Document Type

Article

Publication Date

2025

Notes/Citation Information

© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).

Digital Object Identifier (DOI)

https://doi.org/10.3390/cancers17050734

Funding Information

The research reported in this publication was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health, grant number R01NS107548 (BB); the National Center for Advancing Translational Sciences of the National Institutes of Health, grant number TL1TR001997 (LTR); and the Northern Kentucky/Greater Cincinnati UK Alumni Club Fellowship (LTR). The content is solely the authors’ responsibility and does not necessarily represent the official views of the NINDS or the NIH. This research was also supported by the Biospecimen Procurement and Translational Pathology and Small Animal Imaging Facility Shared Resource Facilities of the University of Kentucky Markey Cancer Center (P30CA177558) and the University of Kentucky Light Microscopy Core and Magnetic Resonance Imaging and Spectroscopy Center.

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