It was hypothesized that the catalyst nanoceria can increase oxidative
stress/inflammation from the basal state and reduce it from the elevated state .
Nanoceria are cleared by macrophages. To test the hypothesis, M0 (non-polarized),
M1- (classically activated, pro-inflammatory), and M2-like (alternatively activated,
regulatory phenotype) RAW 264.7 macrophages were nanoceria exposed. Responses
were quantified by arginase activity, IL-1ß level, cell oxygen consumption rate (OCR),
the glycolysis stress test (GST), morphology determined by light microscopy,
macrophage phenotype marker expression and morphology using a novel three
dimensional immunohistochemical method, and RT-qPCR. Nanoceria blocked
arginase and IL-1ß effects, increased M0 cell OCR and GST toward the M2 phenotype
and altered multiple M1- and M2-like cell endpoints toward the M0 level. M1-like cells
had greater volume and less circularity/roundness, and the M2-like cells had greater
volume than M0 macrophages. Nanoceria converted M1- and M2-like cells toward M0
morphology. The results are overall consistent with the hypothesis.
Digital Object Identifier (DOI)
This work was supported by the National Institutes of Health [grant number R01GM109195]. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health
Yokel, Robert; Ensor, Marsha L.; Vekaria, Hemendra J.; Sullivan, Patrick G.; Feola, David J.; Stromberg, Arnold; Tseng, Michael T.; and Harrison, Douglas A., "Cerium dioxide, a Jekyll and Hyde nanomaterial, can increase basal and decrease elevated inflammation and oxidative stress" (2022). Pharmaceutical Sciences Faculty Publications. 206.