Title
Molecular and Biochemical Signaling Underlying Arabidopsis-Bacterial/Virus/Fungal Interactions
Year of Publication
2016
Degree Name
Doctor of Philosophy (PhD)
Document Type
Doctoral Dissertation
College
Agriculture, Food and Environment
Department
Plant Pathology
First Advisor
Dr. Pradeep Kachroo
Abstract
Systemic acquired resistance (SAR) is a form of inducible defense response triggered upon localized infection that confers broad-spectrum disease resistance against secondary infections. Several factors are known to regulate SAR and these include phenolic phytohormone salicylic acid (SA), phosphorylated sugar glycerol-3-phosphate (G3P), and dicarboxylic acid azelaic acid (AzA). This study evaluated a role for free radicals nitric oxide (NO) and reactive oxygen species (ROS) in SAR. Normal accumulation of both NO and ROS was required for normal SAR and mutations preventing NO/ROS accumulation and/or biosynthesis compromised SAR. A role for NO and ROS was further established using pharmacological approaches. Notably, both NO and ROS conferred SAR in a concentration dependent manner. This was further established using genetic mutants that accumulated high levels of NO. NO/ROS acted upstream of G3P and in parallel to SA. Collectively, these results suggest that NO and ROS are essential components of the SAR pathway.
Digital Object Identifier (DOI)
http://dx.doi.org/10.13023/ETD.2016.263
Recommended Citation
El-Shetehy, Mohamed H., "Molecular and Biochemical Signaling Underlying Arabidopsis-Bacterial/Virus/Fungal Interactions" (2016). Theses and Dissertations--Plant Pathology. 19.
https://uknowledge.uky.edu/plantpath_etds/19
Included in
Bacteriology Commons, Biochemistry Commons, Biotechnology Commons, Molecular Biology Commons, Virology Commons