Abstract
Deficiency of ACE2 in macrophages has been suggested to promote the development of an inflammatory M1 macrophage phenotype. We evaluated effects of ACE2 deficiency in bone-marrow-derived stem cells on adipose inflammation and glucose tolerance in C57BL/6 mice fed a high fat (HF) diet. ACE2 activity was increased in the stromal vascular fraction (SVF) isolated from visceral, but not subcutaneous adipose tissue of HF-fed mice. Deficiency of ACE2 in bone marrow cells significantly increased mRNA abundance of F4/80 and TNF-α in the SVF isolated from visceral adipose tissue of HF-fed chimeric mice, supporting increased presence of inflammatory macrophages in adipose tissue. Moreover, deficiency of ACE2 in bone marrow cells modestly augmented glucose intolerance in HF-fed chimeric mice and increased blood levels of glycosylated hemoglobin. In summary, ACE2 deficiency in bone marrow cells promotes inflammation in adipose tissue and augments obesity-induced glucose intolerance.
Document Type
Article
Publication Date
2012
Digital Object Identifier (DOI)
http://dx.doi.org/10.1155/2012/762094
Repository Citation
Thatcher, Sean E.; Gupte, Manisha; Hatch, Nicholas; and Cassis, Lisa A., "Deficiency of ACE2 in Bone-Marrow-Derived Cells Increases Expression of TNF-α in Adipose Stromal Cells and Augments Glucose Intolerance in Obese C57BL/6 Mice" (2012). Graduate Center for Nutritional Sciences Faculty Publications. 8.
https://uknowledge.uky.edu/nutrisci_facpub/8
Notes/Citation Information
Published in International Journal of Hypertension, v. 2012, article ID 762094, p. 1-8.
Copyright © 2012 Sean E. Thatcher et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.