Adaptation of Microelectrode Array Technology for the Study of Anesthesia-Induced Neurotoxicity in the Intact Piglet Brain

Authors

Emily D. Geyer, The Ohio State University
Prithvi A. Shetty, The Ohio State University
Christopher J. Suozzi, The Ohio State University
David Z. Allen, The Ohio State University
Pamela P. Benavidez, The Ohio State University
Joseph Liu, The Ohio State University
Charles N. Hollis, The Ohio State University
Greg A. Gerhardt, University of KentuckyFollow
Jorge E. Quintero, University of KentuckyFollow
Jason J. Burmeister, University of Kentucky
Emmett E. Whitaker, The Ohio State University

Abstract

Every year, millions of children undergo anesthesia for a multitude of procedures. However, studies in both animals and humans have called into question the safety of anesthesia in children, implicating anesthetics as potentially toxic to the brain in development. To date, no studies have successfully elucidated the mechanism(s) by which anesthesia may be neurotoxic. Animal studies allow investigation of such mechanisms, and neonatal piglets represent an excellent model to study these effects due to their striking developmental similarities to the human brain.

This protocol adapts the use of enzyme-based microelectrode array (MEA) technology as a novel way to study the mechanism(s) of anesthesia-induced neurotoxicity (AIN). MEAs enable real-time monitoring of in vivo neurotransmitter activity and offer exceptional temporal and spatial resolution. It is hypothesized that anesthetic neurotoxicity is caused in part by glutamate dysregulation and MEAs offer a method to measure glutamate. The novel implementation of MEA technology in a piglet model presents a unique opportunity for the study of AIN.

Document Type

Article

Publication Date

5-12-2018

Notes/Citation Information

Published in Journal of Visualized Experiments, issue 135, e57391, p. 1-12.

Creative Commons Attribution 3.0 License

Digital Object Identifier (DOI)

https://doi.org/10.3791/57391

Related Content

The video component of this article can be found at https://www.jove.com/video/57391/.

Repository Citation

Geyer, Emily D.; Shetty, Prithvi A.; Suozzi, Christopher J.; Allen, David Z.; Benavidez, Pamela P.; Liu, Joseph; Hollis, Charles N.; Gerhardt, Greg A.; Quintero, Jorge E.; Burmeister, Jason J.; and Whitaker, Emmett E., "Adaptation of Microelectrode Array Technology for the Study of Anesthesia-Induced Neurotoxicity in the Intact Piglet Brain" (2018). Neuroscience Faculty Publications. 46.
https://uknowledge.uky.edu/neurobio_facpub/46