Abstract
Data in this article are associated with the research article “Highthroughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins” (Yang et al., 2017) [1]. This article provided data on how to develop an immunoturbidimetric assay (ITA) of enzyme/mutants as proteins in cell lysates in high-throughput (HTP) mode together with HTP assay of their activities to derive their specific activities in cell lysates for comparison, with Pseudomonas aeruginosa arylsulfatase (PAAS) and Bacillus fastidious uricase (BFU) plus their mutants as models. Data were made publicly available for further analyses.
Document Type
Article
Publication Date
7-15-2017
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.dib.2017.07.019
Funding Information
This work was supported in part by the National Natural Science Foundation of China, China (Nos. 31570862, and 21402108), and the Ministry of Education of China (No. 20125503110007).
Related Content
Transparency data document associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2017.07.019.
Repository Citation
Feng, Yiran; Yang, Xiaolan; Chong, Huimin; Wang, Deqiang; Hu, Xiaolei; Zhan, Chang-Guo; and Liao, Fei, "Data for High-Throughput Estimation of Specific Activities of Enzyme/Mutants in Cell Lysates Through Immunoturbidimetric Assay of Proteins" (2017). Molecular Modeling and Biopharmaceutical Center Faculty Publications. 10.
https://uknowledge.uky.edu/mmbc_facpub/10
Transparency document. Supplementary material
Included in
Amino Acids, Peptides, and Proteins Commons, Enzymes and Coenzymes Commons, Pharmacy and Pharmaceutical Sciences Commons
Notes/Citation Information
Published in Data in Brief, v. 14, p. 220-245.
© 2017 The Author(s). Published by Elsevier Inc.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).