Abstract
The human pathogen L. monocytogenes and the animal pathogen L. ivanovii, together with four other species isolated from symptom-free animals, form the "Listeria sensu stricto" clade. The members of the second clade, "Listeria sensu lato", are believed to be solely environmental bacteria without the ability to colonize mammalian hosts. To identify novel determinants that contribute to infection by L. monocytogenes, the causative agent of the foodborne disease listeriosis, we performed a genome comparison of the two clades and found 151 candidate genes that are conserved in the Listeria sensu stricto species. Two factors were investigated further in vitro and in vivo. A mutant lacking an ATP-binding cassette transporter exhibited defective adhesion and invasion of human Caco-2 cells. Using a mouse model of foodborne L. monocytogenes infection, a reduced number of the mutant strain compared to the parental strain was observed in the small intestine and the liver. Another mutant with a defective 1,2-propanediol degradation pathway showed reduced persistence in the stool of infected mice, suggesting a role of 1,2-propanediol as a carbon and energy source of listeriae during infection. These findings reveal the relevance of novel factors for the colonization process of L. monocytogenes.
Document Type
Article
Publication Date
12-19-2017
Digital Object Identifier (DOI)
https://doi.org/10.1038/s41598-017-17570-0
Funding Information
This work was supported by a grant to JS within the Research Training Group (Graduiertenkolleg) 1482 of the Deutsche Forschungsgemeinschaft.
Related Content
Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-017-17570-0.
Repository Citation
Schardt, Jakob; Jones, Grant; Müller-Herbst, Stefanie; Schauer, Kristina; D'Orazio, Sarah E. F.; and Fuchs, Thilo M., "Comparison Between Listeria sensu stricto and Listeria sensu lato Strains Identifies Novel Determinants Involved in Infection" (2017). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 99.
https://uknowledge.uky.edu/microbio_facpub/99
Supplementary Information
Included in
Bacteria Commons, Medical Immunology Commons, Medical Microbiology Commons, Molecular Genetics Commons, Pathogenic Microbiology Commons
Notes/Citation Information
Published in Scientific Reports, v. 7, article no. 17821, p. 1-14.
© The Author(s) 2017
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