Abstract
BACKGROUND: Idiopathic pulmonary arterial hypertension (IPAH) continues to be one of the most serious intractable diseases that might start with activation of several triggers representing the genetic susceptibility of a patient. To elucidate what essentially contributes to the onset and progression of IPAH, we investigated factors playing an important role in IPAH by searching discrepant or controversial expression patterns between our murine model and those previously published for human IPAH. We employed the mouse model, which induced muscularization of pulmonary artery leading to hypertension by repeated intratracheal injection of Stachybotrys chartarum, a member of nonpathogenic and ubiquitous fungus in our envelopment.
METHODS: Microarray assays with ontology and pathway analyses were performed with the lungs of mice. A comparison was made of the expression patterns of biological pathways between our model and those published for IPAH.
RESULTS: Some pathways in our model showed the same expression patterns in IPAH, which included bone morphogenetic protein (BMP) signaling with down-regulation of BMP receptor type 2, activin-like kinase type 1, and endoglin. On the other hand, both Wnt/planar cell polarity (PCP) signaling and its downstream Rho/ROCK signaling were found alone to be activated in IPAH and not in our model.
CONCLUSIONS: Activation of Wnt/PCP signaling, in upstream positions of the pathway, found alone in lungs from end stage IPAH may play essential roles in the pathogenesis of the disease.
Document Type
Article
Publication Date
10-17-2012
Digital Object Identifier (DOI)
http://dx.doi.org/10.1186/1465-9921-13-103
Repository Citation
Shimodaira, Kayoko; Okubo, Yoichiro; Ochiai, Eri; Nakayama, Haruo; Katano, Harutaka; Wakayama, Megumi; Shinozaki, Minoru; Ishiwatari, Takao; Sasai, Daisuke; Tochigi, Naobumi; Nemoto, Tetsuo; Saji, Tsutomu; Kamei, Katsuhiko; and Shibuya, Kazutoshi, "Gene Expression Analysis of a Murine Model with Pulmonary Vascular Remodeling Compared to End-Stage IPAH Lungs" (2012). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 24.
https://uknowledge.uky.edu/microbio_facpub/24
Notes/Citation Information
Published in Respiratory Research, v. 13, 103.
© 2012 Shimodaira et al.; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.