Priming of Peripheral Lymph Node B Cells with TNP-Ficoll: Role of Lymphokines in B Cell Differentiation

Authors

S. Niranjan Goud, University of Kentucky
Alan M. Kaplan, University of KentuckyFollow
Subbarao Bondada, University of KentuckyFollow

Abstract

We have previously shown that peripheral lymph node (PLN) B lymphocytes of adult DBA/ 25 mice failed to make an antibody responseto type 2 antigen TNP-Ficoll, but exhibited a good antibody response to type 1 antigen TNP-BruceNa abortus. In the present study we wanted to find out whether the unresponsivenessof PLN B cells to TNP-Ficoll is due to defectsin the early activation and proliferation stage or in the final differentiation stageof B cells. Therefore, we have used a two-step protocol of in vivo immunization of mice with TNP-Ficoll and the subsequent in vitro challenge with TNP-Brucella abortus and studied the anti-TNP plaque-forming cell (PFC) responses.The results indicate a three- to sixfold increase of PFC responsesin PLN cell cultures derived from TNI-Ficoll-primed animals compared to saline control mice. This increasedantibody responsewas TNP-specific as 93% of the PFC’s were inhibited by TNP-lysine. Limiting dilution experiments confirm that the increase in anti-TNP PFC response from the TNP-Ficoll-primed animals was indeed due to an increase in TNP-specific precursor B cells. Further, the addition of rIL-5 or rIL-6 induced anti-TNP PFC in the TNP-Ficoll-primed and in control PLN cell cultures in the presence of antigen. However, in primed PLN cells lymphokines alone were sufficient to restore anti-TNP PFC response. In conclusion, our results show that in PLN, the TNP-Ficoll can induce proliferation of hapten-specific B cells but not final differentiation. These primed PLN B cells mature into antibody-secreting cells upon stimulation with TNP-BA or lymphokines.

Document Type

Article

Publication Date

1992

Notes/Citation Information

© 1992 Academic Press. Inc.

Digital Object Identifier (DOI)

https://doi.org/10.1016/0008-8749(92)90255-N

Funding Information

This work was supported by a grant from the University of Kentucky Tobacco and Health ResearchInstitute, and by the NIH Grants AI 121490, AGO5731, and a ResearchCareer Development Award K04 AGO0422 from the National Institute of Health to B.S.

Repository Citation

Goud, S. Niranjan; Kaplan, Alan M.; and Bondada, Subbarao, "Priming of Peripheral Lymph Node B Cells with TNP-Ficoll: Role of Lymphokines in B Cell Differentiation" (1992). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 195.
https://uknowledge.uky.edu/microbio_facpub/195