Abstract
The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2 resembles BSF-1 in its ability to activate small resting B cells and enhancement of surface Ia. Anti-Lyb-2 antibodies bound B cells with very high avidity and were able to induce mobilization of cytosolic-free calcium. Anti-Lyb-2 mAB differs from BSF-1 in that BSF-1 but not anti-Lyb-2 is able to synergize with anti-μ in induction of B-cell proliferation. The relation between Lyb-2 molecules and BSF-1 receptors was tested in assays that measure binding of anti-Lyb-2 or BSF-1 in B cells and were found not to compete with each other. It appears that the two B-cell agonists anti-Lyb-2 and BSF-1 may exert their effects on B cells through different cell surface moieties as well as different intracellular pathways.
Document Type
Article
Publication Date
1988
Digital Object Identifier (DOI)
https://doi.org/10.1016/0008-8749(88)90302-4
Funding Information
This work was supported in part by NIH Grants AI 2 1490 and AC 0753 1 and by an award from the Tobacco and Health Research Institute of the University of Kentucky. A. R. Baluyut is supported by NIH Training Grant CA 09509.
Repository Citation
Bondada, Subbarao; Morris, Joan; and Baluyut, A. R., "Properties of Anti-Lyb-2-Mediated B-Cell Activation and the Relationship between Lyb-2 Molecules and Receptors for B-Cell Stimulatory Factor-1 on Murine B Lymphocytes" (1988). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 187.
https://uknowledge.uky.edu/microbio_facpub/187
Notes/Citation Information
© 1988 Academic Press, Inc.