Tritium (3H) radiolabeling of protein A and antibody to high specific activity: Application to cell surface antigen radioimmunoassays

Authors

Ronald L. Wilder, National Institute of Allergy and Infectious Diseases
Catherine C. Yuen
Subbarao BondadaFollow
Virgil L. Woods
Cornelius B. Alexander
Rose G. Mage

Abstract

Staphylococcal protein A and several different immunoglobulins have been radio-labeled to high specific activities (> 106 cpm/pg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.

Document Type

Article

Publication Date

1979

Notes/Citation Information

© Elsevier/North-Holland Biomedical Press

Digital Object Identifier (DOI)

https://doi.org/10.1016/0022-1759(79)90192-3

Repository Citation

Wilder, Ronald L.; Yuen, Catherine C.; Bondada, Subbarao; Woods, Virgil L.; Alexander, Cornelius B.; and Mage, Rose G., "Tritium (3H) radiolabeling of protein A and antibody to high specific activity: Application to cell surface antigen radioimmunoassays" (1979). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 183.
https://uknowledge.uky.edu/microbio_facpub/183