Abstract
Leukocyte diversity of the first-trimester maternal-fetal interface has been extensively described; however, the immunological landscape of the term decidua remains poorly understood. We therefore profiled human leukocytes from term decidua collected via scheduled cesarean delivery. Relative to the first trimester, our analyses show a shift from NK cells and macrophages to T cells and enhanced immune activation. Although circulating and decidual T cells are phenotypically distinct, they demonstrate significant clonotype sharing. We also report significant diversity within decidual macrophages, the frequency of which positively correlates with pregravid maternal body mass index. Interestingly, the ability of decidual macrophages to respond to bacterial ligands is reduced with pregravid obesity, suggestive of skewing toward immunoregulation as a possible mechanism to safeguard the fetus against excessive maternal inflammation. These findings are a resource for future studies investigating pathological conditions that compromise fetal health and reproductive success.
Document Type
Article
Publication Date
2023
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.celrep.2023.112769
Funding Information
We are grateful to all participants in this study. We thank the MFM Research Unit at Oregon Health and Science University for sample collection and Allen Jankeel, Michael Z. Zulu, Gouri Ajith, Isaac Cinco, and Hannah Debray at University of California, Irvine (UCI) for assistance with tissue processing. We thank Dr. Jennifer Atwood at the UCI Institute for Immunology Flow Cytometry Core for assistance with FACS sorting, imaging flow cytometry and Dr. Melanie Oakes at the UCI Genomics Research and Technology Hub (GRT Hub) for assistance with 10x library preparation and sequencing. This study was supported by grants from the National Institutes of Health 1K23HD06952 (N.E.M.), 1R01AI145910 (I.M.), R03AI11280 (I.M.), and 1R01AI142841 (I.M.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Repository Citation
Sureshchandra, Suhas; Doratt, Brianna M.; True, Heather E.; Mendoza, Norma; Rincon, Monica; Marshall, Nicole E.; and Messaoudi, Ilhem, "Multimodal profiling of term human decidua demonstrates immune adaptations with pregravid obesity" (2023). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 177.
https://uknowledge.uky.edu/microbio_facpub/177
Notes/Citation Information
© 2023 The Author(s).