Abstract
Analysis of protein sequences by the informational spectrum method (ISM) enables characterization of their specificity according to encoded information represented with defined frequency (F). Our previous data showed that F(0.367) is characteristic for variable heavy chain (VH) domains (a combination of variable (V), diversity (D) and joining (J) gene segments) of the anti-phosphocholine (PC) T15 antibodies and mostly dependent on the CDR2 region, a site for PC phosphate group binding. Because the T15 dsDNA-reactive U4 mutant also encodes F(0.367), we hypothesized that the same frequency may also be characteristic for anti-DNA antibodies. Data obtained from an analysis of 60 spontaneously produced anti-DNA antibody VH domain sequences supported our hypothesis only for antibodies, which use V gene segment in germline configuration, such as S57(VH31), MRL-DNA22, and VH11, members of the VH1 (J558) and VH7 (S107) gene families. The important finding is that out of seven V gene segments used by spontaneous anti-DNA antibodies, F(0.367) is only expressed by the germline configuration of these three V gene segments. The data suggest that antibody specificity for the phosphate group moiety delineated as F(0.367) is the intrinsic property of the V germline gene segments used, whereas paratope/epitope interaction with antigens bearing this epitope, such as PC or dsDNA, requires corresponding antibody VH conformation that is susceptible to somatic mutation(s).
Document Type
Article
Publication Date
10-18-2018
Digital Object Identifier (DOI)
https://doi.org/10.3389/fimmu.2018.02378
Funding Information
This work was supported by a grant from the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 175056).
Related Content
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2018.02378/full#supplementary-material
Repository Citation
Srdic-Rajic, Tatjana; Kohler, Heinz; Jurisic, Vladimir; and Metlas, Radmila, "Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used" (2018). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 136.
https://uknowledge.uky.edu/microbio_facpub/136
Included in
Medical Genetics Commons, Medical Immunology Commons, Medical Microbiology Commons, Molecular Genetics Commons
Notes/Citation Information
Published in Frontiers in Immunology, v. 9, 2378, p. 1-7.
© 2018 Srdic-Rajic, Kohler, Jurisic and Metlas.
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