Abstract
The link between EBV infection and Burkitt lymphoma (BL) is strong, but the mechanism underlying that link has been elusive. We have developed a mouse model for EBV-associated BL in which LMP2A, an EBV latency protein, and MYC are expressed in B cells. Our model has demonstrated the ability of LMP2A to accelerate tumor onset, increase spleen size, and bypass p53 inactivation. Here we describe the results of total gene expression analysis of tumor and pretumor B cells from our transgenic mouse model. Although we see many phenotypic differences and changes in gene expression in pretumor B cells, the transcriptional profiles of tumor cells from LMP2A/λ-MYC and λ-MYC mice are strikingly similar, with fewer than 20 genes differentially expressed. We evaluated the functional significance of one of the most interesting differentially expressed genes, Egr1, and found that it was not required for acceleration of tumor onset by LMP2A. Our studies demonstrate the remarkable ability of LMP2A to affect the pretumor B-cell phenotype and tumorigenesis without substantially altering gene expression in tumor cells.
Document Type
Article
Publication Date
12-22-2011
Digital Object Identifier (DOI)
https://doi.org/10.1182/blood-2011-02-338434
Funding Information
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked ‘‘advertisement’’ in accordance with 18 USC section 1734.
Related Content
The online version of this article contains a data supplement.
Repository Citation
Bieging, Kathryn T.; Fish, Kamonwan; Bondada, Subbarao; and Longnecker, Richard, "A Shared Gene Expression Signature in Mouse Models of EBV-Associated and Non-EBV-Associated Burkitt Lymphoma" (2011). Microbiology, Immunology, and Molecular Genetics Faculty Publications. 132.
https://uknowledge.uky.edu/microbio_facpub/132
Data Supplement: Document 1. Supplemental tables
Included in
Cancer Biology Commons, Medical Immunology Commons, Medical Microbiology Commons, Molecular Genetics Commons
Notes/Citation Information
Published in Blood, v. 118, no. 26, p. 6849-6859.
This research was originally published in Blood. Kathryn T. Bieging, Kamonwan Fish, Subbarao Bondada and Richard Longnecker. A shared gene expression signature in mouse models of EBV-associated and non–EBV-associated Burkitt lymphoma. Blood. 2011;118:6849-6859. © 2011 by The American Society of Hematology.
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