Date Available
7-14-2017
Year of Publication
2017
Document Type
Master's Thesis
Degree Name
Master of Science (MS)
College
Medicine
Department/School/Program
Medical Sciences
Advisor
Dr. M. Paul Murphy
Abstract
Cleaved fragments of the extracellular matrix protein perlecan have been shown to promote neuroprotection and repair after ischemic stroke. The cysteine proteases cathepsin B and L as well as the metalloprotease bone morphogenic protein 1 (BMP-1) are capable of releasing the biologically active C-terminal laminin-like globular domain (LG3) of perlecan. Exercise, a known method of reducing stroke risk and severity, has been shown to increase the expression of some proteases associated with perlecan processing. Using a transient distal middle cerebral artery occlusion (MCAo) model for focal ischemic stroke we show that while 7 days of running only slightly decreased infarct volume, BMP1 and perlecan (HSPG2) RNA expression in skeletal muscle was significantly increased in 3-month-old male wild type C57/BL6 mice. Moreover, elevated levels of BMP1 RNA were still detectable after 3 days of detraining, suggesting a prolonged effect of exercise on BMP1 expression. Levels of LG3 in the blood were below the limit of detection in the current study, however it is likely that a more sensitive method would enable analysis of serum. These preliminary findings suggest that LG3 could be a molecular mediator of neuroprotection afforded by exercise though further studies are required.
Digital Object Identifier (DOI)
https://doi.org/10.13023/ETD.2017.327
Recommended Citation
Grohs, Gillian, "THE EFFECTS OF EXERCISE PRECONDITIONING ON FOCAL ISCHEMIC STROKE" (2017). Theses and Dissertations--Medical Sciences. 8.
https://uknowledge.uky.edu/medsci_etds/8