Date Available

5-19-2023

Year of Publication

2021

Degree Name

Master of Science (MS)

Document Type

Master's Thesis

College

Medicine

Department/School/Program

Medical Sciences

First Advisor

Dr. Christopher Fry

Abstract

ACL tear is a common injury in the United States with a significant cost and time burden during recovery; reconstructive surgery often results in weakened quadricep muscle and higher risk of posttraumatic osteoarthritis. Prior research has highlighted cellular derangements within the quadriceps after ACL injury and reconstruction, but the early molecular effectors of cellular dysfunction remain to be identified. To further understand the molecular effectors of quadriceps dysfunction following ACL injury, this study aims to quantify the timing and magnitude of transcriptional differences between the quadriceps muscle of injured and contralateral non-injured limbs. These data will provide insight into the early molecular events after ACL injury to identify potential treatment points in our pre-clinical ACL injury model. Immunohistochemical images and transcriptional activity assays from mouse quadriceps following surgical ACL transection were taken after one, three, seven, and ten days. These data were compared with their contralateral non-injured limb. Results showed early myonuclear transcriptional differences precede discernable atrophy. These data provide a time course of early molecular changes after ACL injury that will aid in optimizing the delivery of therapeutics to mitigate or rescue deficits within the injury limb muscle.

Digital Object Identifier (DOI)

https://doi.org/10.13023/etd.2021.138

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