Year of Publication

2019

Degree Name

Master of Science (MS)

Document Type

Master's Thesis

College

Medicine

Department

Medical Sciences

First Advisor

Dr. Greg A. Gerhardt

Abstract

Nitric Oxide (NO) is a powerful endogenous free radical that has numerous biological functions including vasodilation and serves as a post synaptic second messenger in the central nervous system (CNS). Numerous studies implicate NO• involvement in CNS disorders such as schizophrenia and drug abuse. These studies address the direct in vivo determination of an FDA-approved NO• donor (nitroglycerin) on extracellular levels of NO• in the frontal cortex and core of the nucleus accumbens in a lightly anesthetized rat. State-of-the-art in vivo amperometric recording techniques coupled with a novel 4-channel low noise pre-amplifier system and new generation microelectrode arrays (MEAs) will be used to record extracellular levels of NO• at 100Hz before and during nasal administration of either placebo (1) or nitroglycerin. This studies will determine the feasibility of measuring NO• in the CNS while administering the NO• donor nasally and determine the amplitude and kinetic time course effects of a nasally delivered NO• donor in two rat brain areas, the frontal cortex and core of the nucleus accumbens.

Digital Object Identifier (DOI)

https://doi.org/10.13023/etd.2019.235

Funding Information

This research was funded by BDD Berlin Drug Development GmbH.

Available for download on Friday, June 11, 2021

Share

COinS