Track 4-1-1: Plant Genetic Resources, Collection, Conversation, Evaluation and IPR Issues

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Publication Date

2015

Location

New Delhi, India

Description

Enzymatic and non-enzymatic antioxidation systems provide protection against the toxic effects of activated oxygen species including scavenging H2O2. It is evident that during stress plants produce different ROS which play a negative role in regulating the antioxidant system and a positive role in signaling events that regulate ion channel activity and gene expression Microarray data indicate that ovules and pistils from apomictic Boechera differ in gene expression from those of sexual Boechera. Specifically, these differences involve genes responsible for stress response, RNA processing, ribosome synthesis and function, photosynthesis and cell cycle regulation. Thus, in the present study, activities of three antioxidant enzymes viz. guaiacol peroxidase, catalase and ascorbate peroxidase were measured in the young unopened buds of two sexual (B. stricta and B. yellowstonensis) and two apomictic (B. retrofracta x stricta and B. microphylla). The activity staining of these enzymes were performed to detect isozymes of these enzymes in a species-specific manner.

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Status and Role of Antioxidant Enzymes in Sexual and Apomictic Species of Boechera

New Delhi, India

Enzymatic and non-enzymatic antioxidation systems provide protection against the toxic effects of activated oxygen species including scavenging H2O2. It is evident that during stress plants produce different ROS which play a negative role in regulating the antioxidant system and a positive role in signaling events that regulate ion channel activity and gene expression Microarray data indicate that ovules and pistils from apomictic Boechera differ in gene expression from those of sexual Boechera. Specifically, these differences involve genes responsible for stress response, RNA processing, ribosome synthesis and function, photosynthesis and cell cycle regulation. Thus, in the present study, activities of three antioxidant enzymes viz. guaiacol peroxidase, catalase and ascorbate peroxidase were measured in the young unopened buds of two sexual (B. stricta and B. yellowstonensis) and two apomictic (B. retrofracta x stricta and B. microphylla). The activity staining of these enzymes were performed to detect isozymes of these enzymes in a species-specific manner.