Track 1-03

Description

Cocksfoot (Dactylis glomerata L.) originated from the Northern Hemisphere, including Eurasia and North Africa. It is widely distributed in temperate climates and has been widely used in the world more than 100 years, especially in North America, Europe and Japan. Genetic variability of cocksfoot had been detected by RAPD (Kölliker et al. 1999), and the correlation between genome size of natural populations of cocksfoot and location altitude has been analyzed by AFLP (Reeves et al. 1999). To date, information on the genetic diversity of cocksfoot at the molecular level is still scarce, This study is the first to combine the genetic diversity research of cocksfoot with SRAP (Sequence Related Amplified Polymorphism) and ISSR (inter-simple sequence repeat) markers evaluating genetic diversity among 45 populations of cocksfoot collected from eight different countries from four continents. The objectives of the research were: (1) to evaluate the levels and patterns of genetic diversity about cocksfoot; (2) to compare ISSR and SRAP markers for the molecular characterization of cocksfoot, including the evaluation of the degree of polymorphism generated from each technique as a pre-requisite for (3) their applicability to formulate appropriate strategies for the conservation and utilization of the wild cocksfoot genetic resources available and to discuss scientific breeding measures according to the status of cultivars.

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Genetic Diversity and Relationships in Cocksfoot by Molecular Markers

Cocksfoot (Dactylis glomerata L.) originated from the Northern Hemisphere, including Eurasia and North Africa. It is widely distributed in temperate climates and has been widely used in the world more than 100 years, especially in North America, Europe and Japan. Genetic variability of cocksfoot had been detected by RAPD (Kölliker et al. 1999), and the correlation between genome size of natural populations of cocksfoot and location altitude has been analyzed by AFLP (Reeves et al. 1999). To date, information on the genetic diversity of cocksfoot at the molecular level is still scarce, This study is the first to combine the genetic diversity research of cocksfoot with SRAP (Sequence Related Amplified Polymorphism) and ISSR (inter-simple sequence repeat) markers evaluating genetic diversity among 45 populations of cocksfoot collected from eight different countries from four continents. The objectives of the research were: (1) to evaluate the levels and patterns of genetic diversity about cocksfoot; (2) to compare ISSR and SRAP markers for the molecular characterization of cocksfoot, including the evaluation of the degree of polymorphism generated from each technique as a pre-requisite for (3) their applicability to formulate appropriate strategies for the conservation and utilization of the wild cocksfoot genetic resources available and to discuss scientific breeding measures according to the status of cultivars.