Track 1-03

Description

RNA-Seq, a massively parallel sequencing method for transcriptome analysis, only analyzes transcribed portions of the genome. Recently, RNA-Seq has provided an opportunity to expand the identification of alfalfa (Medicago sativa) genes. Using Illumina sequencing, 124,025 unique sequences from MSGI 1.0 have been identified from the elongating stem and post-elongation stem internodes of two alfalfa genotypes (Yang et al. 2011). Using 454 sequencing, 54,216 unique sequences were obtained from the roots and shoots of two alfalfa genotypes (Han et al., 2011). In addition, Illumina sequencing of old and young stems of 27 alfalfa genotypes led to the identification of 25,183 contigs (Li et al. 2012). While these experiments have identified numerous transcripts, the transcripts were derived only from stems, roots, and shoots. Therefore, further transcriptome sequencing of a broader array of tissues permit the global identification of transcripts that would be useful in modern alfalfa breeding programs.

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Global Illumina Sequencing and the Development of EST-SSR Markers in Alfalfa

RNA-Seq, a massively parallel sequencing method for transcriptome analysis, only analyzes transcribed portions of the genome. Recently, RNA-Seq has provided an opportunity to expand the identification of alfalfa (Medicago sativa) genes. Using Illumina sequencing, 124,025 unique sequences from MSGI 1.0 have been identified from the elongating stem and post-elongation stem internodes of two alfalfa genotypes (Yang et al. 2011). Using 454 sequencing, 54,216 unique sequences were obtained from the roots and shoots of two alfalfa genotypes (Han et al., 2011). In addition, Illumina sequencing of old and young stems of 27 alfalfa genotypes led to the identification of 25,183 contigs (Li et al. 2012). While these experiments have identified numerous transcripts, the transcripts were derived only from stems, roots, and shoots. Therefore, further transcriptome sequencing of a broader array of tissues permit the global identification of transcripts that would be useful in modern alfalfa breeding programs.